Thursday, June 28, 2012

Circumcising a healthy infant goes against the Hippocratic Oath


ATCC® Primary Cell Solutions™
ATCC® Number:PCS-110-010    Price:$559.00 (for-profit list price)
$559.00 (non-profit list price)
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Name:Primary Dermal Microvascular Endothelial Cells; Normal, Human, Neonatal
Biosafety Level:1
Organism:Homo sapiens (human)
Source:Neonatal foreskin
Growth Properties:Adherent
Morphology:Cobblestone appearance with large dark nuclei; during proliferation, cells are small and evenly sized, display a high mitotic index and show no presence of smooth muscle cells.
Volume per Vial:1 ml
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Vascular Cell Basal Medium (ATCC PCS-100-030)
Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040)
Microvascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-110-041)
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200)
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003)
Trypsin Neutralizing Solution (ATCC PCS-999-004)
Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
Phenol Red (ATCC PCS-999-001)
Recomended Culture Conditions:See Product Information Sheet
Comments:The cells are cryopreserved in the third passage to ensure the greatest purity of the cell population, coupled with high viability and plating efficiency. 
A complete solution to propagate dermal microvascular cells in low serum (5% FBS) conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Microvascular Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired.
Applications:Drug screening, angiogenesis, oncology, inflammation, intercellular communication
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests:Bacteria and Yeast: Negative
Mycoplasma: Negative
Testing:Cells are von Willebrand factor positive and smooth muscle α-actin negative.

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